解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Death-associated protein (DAP)-kinase, an actin-cytoskeleton localized serine/threonine kinase, functions as a novel tumor suppressor and participates in a wide variety of cell death systems. Recent studies indicate that DAP-kinase elicits a potent cytoskeletal reorganization effect and is capable of modulating integr...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1007/s11373-005-9063-5
更新日期:2006-03-01 00:00:00
abstract::The CDC4 gene of Saccharomyces cerevisiae encodes an essential function that is required for G1-S and G2-M transitions during mitosis and at various stages during meiosis. We have isolated a functional homologue of CDC4 (CaCDC4) from the pathogenic yeast Candida albicans by complementing the S. cerevisiae cdc4-3 mutat...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9027-9
更新日期:2005-12-01 00:00:00
abstract::Mutations at codon 215 of HIV-1 reverse transcriptase (RT) confer resistance to nucleoside analogs through RT-catalyzed ATP-dependent phosphorolysis. We showed that mutation T215Y is predominant over T215F (respectively 38.8 vs. 7.04% of 7312 sequences from a cohort of patients receiving antiretroviral therapy in Fran...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-9011-4
更新日期:2005-10-01 00:00:00
abstract::Diazoxide is a selective mitochondria ATP-sensitive potassium (K(ATP)) channel opener, which has been reported to preserve the microvascular integrity of ischemia-reperfusion (I/R)-injured tissues. Our study aimed to assess diazoxide's effects on I/R-injured cremaster muscles and to further elucidate its underlying me...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-3730-4
更新日期:2005-01-01 00:00:00
abstract::Critical assessment of experimental muscle-pain models resulting from maximal muscle contraction may provide a means of assessing hypersensitivity and the central nociceptive mechanisms involved in diffused muscle pain. The aim of the present study was, therefore, to investigate the patterns of nociceptive behavior an...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-6595-7
更新日期:2005-01-01 00:00:00
abstract::Upon cell adhesion to extracellular matrix proteins, focal adhesion kinase (FAK) rapidly undergoes autophosphorylation on its Tyr-397 which consequently serves as a binding site for the Src homology 2 domains of the Src family protein kinases and several other intracellular signaling molecules. In this study, we have ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-7212-5
更新日期:2005-01-01 00:00:00
abstract::The polymeric immunoglobulin receptor (PIGR) mediates transport of IgA and IgM antibodies across mucosal and glandular epithelia. Several studies have utilized immunohistochemistry to demonstrate that PIGR expression varies in different types of lung carcinoma, and is down-regulated during tumor progression. We have p...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-004-8185-5
更新日期:2005-01-01 00:00:00
abstract::The effects of N omega-nitro-L: -arginine (L: -NOARG) and N(G)-monomethyl-L: -arginine (L: -NMMA) on the spatial distribution of diaphragmatic microvascular blood flow were assessed in anesthetized, mechanically ventilated rats. Microvascular blood flow was measured after different periods at either a fixed site (Q st...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-004-8166-8
更新日期:2005-01-01 00:00:00
abstract::The signaling pathways leading to extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) activation by N-formyl-Met-Leu-Phe (fMLP) or platelet activating factor (PAF) in human neutrophils were examined. Previously, we found that changes of intracellular Ca2+ ([Ca2+]i) stimulated by...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-1704-1
更新日期:2005-01-01 00:00:00
abstract::DNA vaccines have emerged as an attractive approach for generating antigen-specific immunotherapy. Strategies that enhance antigen presentation may potentially be used to enhance DNA vaccine potency. Previous experiments showed that chimeric DNA vaccines utilizing endoplasmic reticulum (ER) chaperone molecules, such a...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/s11373-005-0334-y
更新日期:2005-01-01 00:00:00
abstract::Dextromethorphan (DM), an antitussive agent, has been claimed to have anti-inflammatory and immunomodulatory effects in vitro. Thus, the aim of this study was to evaluate the effects of DM on sepsis induced by intravenous (i.v.) administration of lipopolysaccharide (LPS) in anesthetized Wistar rats and by intraperiton...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254358
更新日期:2004-11-01 00:00:00
abstract::Although hypercholesterolemia is a well-known risk factor for atherosclerosis, little is known about the effect of hypercholesterolemia on cardiac contractile function. The objective of this study was to examine the effect of hypercholesterolemia on myocardial contractility. Fifteen New Zealand white rabbits were fed ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254368
更新日期:2004-11-01 00:00:00
abstract::The antitumor activity of Anoectochilus formosanus Hayata (Orchidaceae), a popularly used folk medicine in the treatment of cancers in Asia, was investigated in MCF-7 human mammary carcinoma cells. Plant extracts of A. formosanus were observed to induce apoptosis of MCF-7 cells as evidenced by cell-morphological chang...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000081840
更新日期:2004-11-01 00:00:00
abstract::Diabetes is a multifactorial disease that has now been recognized to involve overproduction of reactive oxygen species and pro-inflammatory cytokines. Peroxisomes are subcellular organelles with several important metabolic functions, and their role in the regulation of cellular oxidative stress is now well established...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000079667
更新日期:2004-09-01 00:00:00
abstract::In this study, we examined the postnatal expression patterns of p63 and other keratinocyte stem cell markers in the rat cornea in an attempt to determine the markers that best represent characteristics of corneal keratinocyte stem cells. We show that the expression of p63 in the rat cornea is unique and differs from t...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256130
更新日期:2004-09-01 00:00:00
abstract::To determine the involvement of the globus pallidus in mediating epilepsy, the effects of microinjection of a GABA uptake blocker (tiagabine), a benzodiazepine agonist (zolpidem) and a GABA-B receptor agonist (baclofen) on pentylenetetrazol (PTZ)-induced tonic seizure were examined in adult rats. Administration of PTZ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256094
更新日期:2004-07-01 00:00:00
abstract::Plasma nitric oxide (NO) levels in Duchenne muscular dystrophy (DMD) patients were significantly lower than those observed in both healthy controls and in patients with other neuromuscular disorders. The correlation between NO level and ejection fraction was significant (r = -0.384, p = 0.0391) in the DMD group. Disru...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256104
更新日期:2004-07-01 00:00:00
abstract::Drosophila eye development is a progressive process including cell fate determination, pattern formation, and rhabdomere morphogenesis. During eye development, a dramatic change in cell shape, which involves turning and extension of the photoreceptor apical surface, occurs in the early pupal stages. It is known that a...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02254441
更新日期:2004-05-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) is a putative anti-stress agent and stress is associated with the secretion of catecholamine from the adrenal gland, but the effects of DHEA on catecholamine secretion are not fully understood. Using bovine chromaffin cells, we found that DHEA inhibited catecholamine secretion and cytosol...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256563
更新日期:2004-03-01 00:00:00
abstract::Chronic inflammation may lead to endothelial dysfunction, which manifests as an impaired coronary reactivity. Impairment in coronary flow reserve (CFR), preceding the clinical symptoms of coronary artery disease, can be measured noninvasively by positron emission tomography. Myeloperoxidase (MPO) is an oxidative enzym...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256549
更新日期:2004-01-01 00:00:00
abstract::Mediation of the respiratory reflex effects of an exogenous serotonin challenge goes beyond the lung vagi and is suggested to involve the nodose ganglia. In the present experiments the effects of an intravenous serotonin challenge on breathing pattern were studied in 8 pentobarbitone-chloralose anaesthetised cats. Bol...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256323
更新日期:2003-11-01 00:00:00
abstract::We previously reported that transgenic ablation of gonadotrophs results in impaired development of cells immunostainable for prolactin (PRL) but not of cells immunostainable for growth hormone (GH) or pro-opiomelanocortin (POMC) in pituitary of newborn mice. The question remained whether this reduction in PRL protein ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000073968
更新日期:2003-11-01 00:00:00
abstract::A number of infectious agents have been implicated in the development of vascular diseases such as atherosclerosis and posttransplantation arterial restenosis. Cytomegalovirus (CMV) has been reported to cause obliteration of coronary arteries by a progressive vasculopathy that involves proliferation of medial smooth m...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256111
更新日期:2003-09-01 00:00:00
abstract::The matrin 3 family of nuclear proteins consists of members with potentially diverse activities. Matrin 3 and NP220 share RNA-binding domains, and NP220 has been shown to recognize and bind to the DNA sequence, CCCCC (G/C). We have isolated and characterized another member of the matrin 3 family, designated NP94, from...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256432
更新日期:2003-07-01 00:00:00
abstract::Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into various tissue types, including bone, cartilage, tendon, adipocytes, and marrow stroma, making them potentially useful for human cell and gene therapies. Our objective was to demonstrate the utility of glass needle-mediated microinj...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256452
更新日期:2003-05-01 00:00:00
abstract::The Peutz-Jeghers syndrome (PJS) is a hereditary disorder that predisposes an individual to benign and malignant tumors in multiple organ systems. Recently, the locus responsible for PJS was mapped genetically to the LKB1 gene, with a subsequent investigation proving that it is responsible for most cases of PJS. LKB1 ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256059
更新日期:2003-03-01 00:00:00
abstract::Glycine N-methyltransferase (GNMT) is a protein with multiple functions. Recently, two Italian siblings who had hepatomegaly and chronic elevation of serum transaminases were diagnosed to have GNMT deficiency caused by inherited compound heterozygosity of the GNMT gene with missence mutations. To evaluate the expressi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256001
更新日期:2003-01-01 00:00:00
abstract::Acute respiratory distress syndrome (ARDS) can be associated with various disorders. Among these, coronavirus infection may cause life-threatening severe acute respiratory syndrome (SARS). In this review, we present animal models and techniques for the study of ARDS, and discuss the roles and possible mechanisms of va...
journal_title:Journal of biomedical science
pub_type: 杂志文章,评审
doi:10.1159/000073523
更新日期:2003-01-01 00:00:00
abstract::The goal of this study was to evaluate the effect of chronic Zn2+ administration (1 mg/kg/day for 1 month) in Sprague-Dawley rats (n = 11) on motility and rearing behaviors (number of events/10 min measured in motility cage), on memory (percentage of failures using a foot-shock double T maze), on the number of muscari...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000067296
更新日期:2002-11-01 00:00:00
abstract::Due to high prevalence and mortality and the lack of effective therapies, prostate cancer is one of the most crucial health problems in men. Drug resistance aggravates the situation, not only in human prostate cancer but also in other cancers. In this study, we report for the first time that cardiac glycosides (e.g. o...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256539
更新日期:2002-09-01 00:00:00
abstract::The detailed mechanism of how lysophosphatidylcholine (LPC) suppresses endothelium-dependent vasodilatation is unclear at present. We investigated the effects of LPC on endothelial intracellular calcium (EC [Ca(2+)](i)) signaling and vascular tone simultaneously using a new technique we developed. Fura-2-labeled rat a...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256588
更新日期:2002-07-01 00:00:00
abstract::Adeno-associated virus (AAV)-2 was developed as a useful vector for human gene therapy. In this report, we analyzed the integration and expression of AAV-mediated ex vivo transferred human beta-globin gene in bone marrow (BM) reconstituted mice. Recombinant AAV (rAAV) containing human beta-globin gene was packaged by ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256072
更新日期:2002-05-01 00:00:00
abstract::The ability of cells of the human monocyte/macrophage lineage to host HIV-1 replication while resisting cell death is believed to significantly contribute to their ability to serve as a reservoir for viral replication in the host. Although macrophages are generally resistant to apoptosis, interruption of anti-apoptoti...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256024
更新日期:2002-03-01 00:00:00
abstract::Permanent functional deficit in patients with spinal cord injury (SCI) is in part due to severe neural cell death. Therefore, cell replacement using stem cells and neural progenitors that give rise to neurons and glia is thought to be a potent strategy to promote tissue repair after SCI. Many studies have shown that s...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256573
更新日期:2002-01-01 00:00:00
abstract::We studied 42 individuals, including 8 patients with either complete or partial syndrome of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS), 8 patients with either complete or partial syndrome of myoclonic epilepsy with ragged-red fibers (MERRF) and 26 maternal family members w...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1159/000064725
更新日期:2002-01-01 00:00:00
abstract::The effects of a single or repeated dermal administration of methyl parathion on motor function, learning and memory were investigated in adult female rats and correlated with blood cholinesterase activity. Exposure to a single dose of 50 mg/kg methyl parathion (75% of the dermal LD(50)) resulted in an 88% inhibition ...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256609
更新日期:2001-11-01 00:00:00
abstract::Adenosine was shown to inhibit norepinephrine (NE) release from sympathetic nerve endings. The purpose of this study was to examine whether endogenous adenosine restrains NE and epinephrine release from the adrenal medulla. The effects of an adenosine receptor antagonist, 1,3-dipropyl-8-(p-sulfophenyl) xanthine (DPSPX...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02255947
更新日期:2001-09-01 00:00:00
abstract::Lysosome-associated membrane protein (LAMP)-1, one of the major protein components of the lysosomal membrane, is upregulated in the human glioblastoma cell lines, U-373 MG and LN-Z308, which undergo cisplatin-induced apoptosis. These human brain tumor cell lines demonstrated apoptosis in response to cisplatin/nifedipi...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02258379
更新日期:2001-07-01 00:00:00
abstract::Our goal was to develop a system to study proteins that associate in vivo with the Moloney murine leukemia virus (M-MuLV) enhancer elements by the isolation of intact proviral chromatin. The M-MuLV long terminal repeats (LTRs) contain tandemly repeated transcriptional enhancer sequences consisting of smaller motifs th...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256602
更新日期:2001-05-01 00:00:00
abstract::Spinal muscular atrophy (SMA) is an autosomal recessive disorder characterized by degeneration of lower motor neurons. We have assayed deletions in two candidate genes, the survival motor neuron (SMN) and neuronal apoptosis inhibitory protein (NAIP) genes, in 108 samples, of which 46 were from SMA patients, and 62 wer...
journal_title:Journal of biomedical science
pub_type: 杂志文章
doi:10.1007/BF02256412
更新日期:2001-03-01 00:00:00